Anti-n-Myc/MYCN antibody [NCM II 100] ab16898 from Abcam

Product Specs
ItemAnti-n-Myc/MYCN antibody [NCM II 100]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab16898
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunoprecipitation
HostMouse, Nude mouse
Quantity50 UG
IsotypeIgG1
ClonalityMonoclonal
CloneNCM II 100
Conjugate/TagUnconjugated
Concentration0.1 mg/ml
Gene Namemycn
ReactivityHuman, Mouse
Targetn-Myc

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Identification of ferroptosis‐related genes in type 2 diabetes mellitus based on machine learningImmunity, Inflammation and DiseaseOctober 11, 2023Sen Wang, Yongpan Lu, Tingting Chi, Yixin Zhang, Yuli Zhao, Huimin Guo, Li Fengtissue sections were incubated with primary antibodies against CD44 (60224‐1‐Ig; Proteintech) and MYCN (ab16898; Abcam) at a dilution of 1:200 at 4°C overnight. On the second day, all sections were incubated with

(2) MYCN driven oncogenesis involves cooperation with WDR5 to activate canonical MYC targets and G9a to repress differentiation genesbioRxivJuly 18, 2023Zhihui Liu, Xiyuan Zhang, Man Xu, Jason J. Hong, Amanda Ciardiello, Haiyan Lei, Jack F. Shern, Carol J. Thieleshaking at 4°C. Two different MYCN antibodies (antibody 1, Santa Cruz, sc-53993; antibody 2, Abcam, ab16898) (4 μg), or normal IgG (4 μg) was incubated with 50 μl Dynabeads M-280 sheep anti-mouse IgG magnetic

(3) CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancerCell death & diseaseMay 4, 2023Wen YC, Tram VTN, Chen WH, Li CH, Yeh HL, Thuy Dung PV, Jiang KC, Li HR, Huang J, Hsiao M, et al.were labeled with 10 ng of an anti-AR antibody (Sigma-Aldrich, 06-680), anti-MYCN antibody (Abcam, ab16898), anti-acetyl-histone H3 antibody (positive control, Novus, NB300-221), or normal rabbit immunoglobulin

(4) Mistletoe lectin inhibits growth of Myc‐amplified small‐cell lung cancerCancer MedicineApril 1, 2023Mohammad A. Shatat, Betsy Gauthier, Suzy Yoon, Eric Yuan, Peiying Yang, Goutham Narla, Afshin Dowlati, Richard T. LeeN‐myc, vinculin, and GAPDH primary antibodies were purchased from Abcam (Waltham, MA, USA. Cat no. ab16898) and Santa Cruz Biotechnology (Cat. no. sc‐73614 and sc‐32233, respectively). All Fluorescence secondary

(5) ARF suppression by MYC but not MYCN confers increased malignancy of aggressive pediatric brain tumorsNature communicationsMarch 3, 2023Mainwaring OJ, Weishaupt H, Zhao M, Rosén G, Borgenvik A, Breinschmid L, Verbaan AD, Richardson S, Thompson D, Clifford SC, et al.# ab203085 (1:1000)), Ki67 [SP6] (Abcam, Cat # ab16667 (1:2000)), n-MYC [NCM II 100] (Abcam, Cat # ab16898 (1:250)), NPR3 (Abcam, Cat # ab97389 (1:250)), Olig-2 (Millipore, Cat # AB9610 (1:1000)), OTX2 (R&D Systems

(6) N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in MedulloblastomaCancer researchJanuary 4, 2023Kuzuoglu-Ozturk D, Aksoy O, Schmidt C, Lea R, Larson JD, Phelps RRL, Nasholm N, Holt M, Contreras A, Huang M, et al.develop the membranes on a Chemidoc imaging system (BioRad). Antibodies used in this study: MYCN (Abcam, ab16898, RRID:AB_443533), p-AKTS473 (Cell Signaling, #4060, RRID:AB_2315049), Total AKT (Cell Signaling, #4685,

(7) The E3 ubiquitin ligase HUWE1 acts through the N‐Myc‐DLL1‐NOTCH1 signaling axis to suppress glioblastoma progressionCancer CommunicationsSeptember 1, 2022Ye Yuan et al.Houston, TX, USA) for 6 h. Then, the cells were collected for co‐immunoprecipitation with anti‐N‐Myc (ab16898, Abcam, Cambridge, MA, UK) followed by Western blotting of ubiquitin. Precipitation with isotype IgG

(8) High temperature induces male sterility via MYB66–MYB4–Casein kinase I signaling in cottonPlant PhysiologyAugust 1, 2022Yanlong Li et al.30-s-on/30-s-off cycles for 15 times until the average chromatin size was approximately 300 bp. Anti-MYC (Abcam, ab16898) antibodies were used to perform immunoprecipitations. Immune complexes were eluted from the protein

(9) p53-Mediated Radiosensitization of (177)Lu-DOTATATE in Neuroblastoma Tumor SpheroidsBiomoleculesNovember 15, 2021Lundsten S, Berglund H, Jha P, Krona C, Hariri M, Nelander S, Lane DP, Nestor M.Waltham, MA, USA), p53 (Abcam ab32389, 1/1000 Cambridge, UK), MDM2 (Abcam ab259265, 1/500), MYCN (Abcam ab16898, 1/250), p21 (Abcam ab109520, 1/1000), Bax (Abcam ab32503, 1/1000), cleaved caspase 3 (Abcam ab13847,

(10) Oncogene-regulated release of extracellular vesiclesDevelopmental CellJuly 1, 2021Seda Kilinc et al.to c-Myc, HRP conjugated",Abcam,Cat# ab205818 Mouse monoclonal anti-n-MYC/MYCN antibody,Abcam,Cat# ab16898; RRID: AB_443533 Rabbit anti-p44/42 MAPK (Erk1/2) antibody,Cell Signaling Technology,Cat# 9102; RRID:

(11) The synergy of BET inhibitors with aurora A kinase inhibitors in MYCN-amplified neuroblastoma is heightened with functional TP53Neoplasia (New York, N.Y.)June 1, 2021Yi JS, Sias-Garcia O, Nasholm N, Hu X, Iniguez AB, Hall MD, Davis M, Guha R, Moreno-Smith M, Barbieri E, et al.overnight at 4°C with the following antibodies: MYCN (Santa Cruz Biotechnologies, sc-56729, or Abcam, ab16898), PARP (CST, 9542S), cleaved Parp (CST, 9541), Vinculin (Santa Cruz Biotechnologies, sc-25336), GAPDH-HRP

(12) Multinational Retrospective Central Pathology Review of Neuroblastoma: Lessons Learned to Establish a Regional Pathology Referral Center in Resource-Limited SettingsArchives of Pathology & Laboratory MedicineFebruary 1, 2021Teresa Santiago et al.validation and optimization process using a mouse monoclonal MYCN antibody (clone NCM II 100, Abcam ab16898) was performed to determine a final optimal dilution of 1:200. A tissue microarray obtained from a cohort

(13) Integrative discovery of treatments for high-risk neuroblastomaNature CommunicationsDecember 1, 2020Elin Almstedt et al.blocked with StartingBlocking T20 TBS (ThermoScientific) and stained with antibodies against the N-Myc (#ab16898, Abcam, 1:250) and with cyclophilin B (#ab16045, Abcam, 1:500) as a loading control. N-Myc protein expression

(14) The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung CancerFrontiers in oncologyJanuary 1, 2020Bian X, Wang X, Zhang Q, Ma L, Cao G, Xu A, Han J, Huang J, Lin W.chromatin was incubated with magnetic beads and antibodies against c-MYC (Abcam, ab32072), MYCN (Abcam, ab16898), BRD4 (Bethyl, A301-985A50), or IgG (Cell Signaling) at room temperature for 6 h. Immunocomplexes were

(15) Hyperglycemia induces spermatogenic disruption via major pathways of diabetes pathogenesisScientific ReportsDecember 1, 2019Constanze C. Maresch, Dina C. Stute, Thomas Fleming, Jihong Lin, Hans-Peter Hammes, Thomas Linn2% BAS in PBS for 1 hour. Then sections were incubated with rabbit anti-PKC antibody (diluted 1:200, ab16898, Abcam, UK) and mouse anti-alpha smooth muscle actin (α-SMA) (diluted 1:500, K046, Linaris, Germany)

(16) SEMA6D regulates perinatal cardiomyocyte proliferation and maturation in miceDevelopmental biologyAugust 1, 2019Sun Q, Peng Y, Zhao Q, Yan S, Liu S, Yang Q, Liu K, Rokosh DG, Jiao K.after images were taken. The primary antibodies used in this study were purchased from Abcam (MYCN #ab16898, α-MHC #ab50967, β-MHC #ab11083, MLC1V #ab680), BD Biosciences (cyclin D1 #556470), Cell Signaling (cleaved

(17) MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastomaOncotargetApril 17, 2018Saurabh Agarwal, Giorgio Milazzo, Kimal Rajapakshe, Ronald Bernardi, Zaowen Chen, Eveline Barbieri, Jan Koster, Giovanni Perini, Cristian Coarfa, Jason M. Shohet(EpiTect ChIP-Grade Antibody Kit (p53), GAH-112, EMD Millipore), and ChIP-grade Anti-MYCN antibody (ab16898, Abcam). Input was generated by purifying DNA from sonicated lysates of each sample. Different gene

(18) Reversible LSD1 inhibition with HCI-2509 induces the p53 gene expression signature and disrupts the MYCN signature in high-risk neuroblastoma cellsOncotargetFebruary 9, 2018Sumati Gupta, Kelly Doyle, Timothy L. Mosbruger, Andrew Butterfield, Alexis Weston, Allison Ast, Mohan Kaadige, Anupam Verma, Sunil Sharma86298S; P53 Rabbit Ab #9282S; LSD1 (C69G12) Rabbit mAb #2184S. Abcam Mouse mAb to n-Myc [NCM II 100] ab16898. siRNA LSD1 SiRNA: Ambion Cat#: 4390827. Negative control Silencer – Negative Control siRNA #1 –

(19) Targeting the MYCN–PARP–DNA Damage Response Pathway in Neuroendocrine Prostate CancerClinical Cancer ResearchFebruary 1, 2018Wei Zhang et al.being blocked by non-fat milk, membranes were incubated with primary antibodies against N-MYC (Abcam, ab16898), PARP1 (Santa Cruz, sc-8007), PARP2 (Abcam, ab176330), TOPBP1 (Abcam, ab2402), BRCA1 (Cell Signaling Technology

(20) Impact of cytosine methylation on DNA binding specificities of human transcription factorsScienceMay 5, 2017Yimeng Yin et al.n-Myc (Abcam cat. no. ab19857 and R&D Systems cat. no. AF1759, AF3158, and AF3640, and Abcam cat. no. ab16898,) in wild-type and defective ES cells or by using antibodies for HOXB13 in transfected LHSAR cells.

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Anti-n-Myc/MYCN antibody [NCM II 100] from Abcam

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Anti-n-Myc/MYCN antibody [NCM II 100] from Abcam

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